Original Research
Development of dried tube specimens for Xpert MTB/RIF proficiency testing
Submitted: 14 January 2020 | Published: 29 September 2020
About the author(s)
Kyle DeGruy, Centers for Disease Control and Prevention, Division of Global HIV and TB, International Laboratory Branch, Atlanta, Georgia, United StatesKatherine Klein, Centers for Disease Control and Prevention, Division of TB Elimination, Laboratory Branch, Atlanta, Georgia, United States
Zilma Rey, Centers for Disease Control and Prevention, Division of Global HIV and TB, International Laboratory Branch, Atlanta, Georgia, United States
Patricia Hall, Centers for Disease Control and Prevention, Division of Global HIV and TB, International Laboratory Branch, Atlanta, Georgia, United States
Andrea Kim, Centers for Disease Control and Prevention, Division of Global HIV and TB, International Laboratory Branch, Atlanta, Georgia, United States
Heather Alexander, Centers for Disease Control and Prevention, Division of Global HIV and TB, International Laboratory Branch, Atlanta, Georgia, United States
Abstract
Background: Proficiency testing (PT) is part of a comprehensive quality assurance programme, which is critical to ensuring patients receive accurate and reliable diagnostic testing. Implementation of the Cepheid Xpert® MTB/RIF assay to aid in the diagnosis of tuberculosis has expanded rapidly in recent years; however, PT material for Xpert MTB/RIF is not readily available in many resource-limited settings.
Objective: To develop an accurate and precise PT material based on the dried tube specimen (DTS) method, using supplies and reagents available in most tuberculosis culture laboratories.
Methods: Dried tube specimens were produced at the United States Centers for Disease Control and Prevention from 2013 to 2015 by inactivating liquid cultures of well-characterised mycobacterial strains. Ten percent of DTS produced were tested with Xpert MTB/RIF and evaluated for accuracy and precision.
Results: Validation testing across eight rounds of PT demonstrated that DTS are highly accurate, achieving an average of 96.8% concordance with the Xpert MTB/RIF results from the original mycobacterial strains. Dried tube specimen testing was also precise, with cycle threshold standard deviations below two cycles when inherent test cartridge variability was low.
Conclusion: Dried tube specimens can be produced using equipment already present in tuberculosis culture laboratories, making Xpert MTB/RIF PT scale-up more feasible in resource-limited settings. Use of DTS may fill the gap in tuberculosis laboratory access to external quality assessment, which is an essential component of a comprehensive continuous quality improvement programme.
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Crossref Citations
1. Advancing proficiency testing for ultra in resource-limited settings using dried tube specimen: A study by SRL-Uganda
Joel Kabugo, Joanita Namutebi, Isa Adam, Dennis Mujuni, Didas Tugumisirize, Oola Denis, George William Kasule, Ivan Ibanda, Beatrice Orena, Henry Byabajungu, Elizabeth Nampewo, Moses Joloba, Yanping Yuan
PLOS ONE vol: 18 issue: 3 first page: e0282650 year: 2023
doi: 10.1371/journal.pone.0282650