Opinion Paper

Challenges and complexities in evaluating severe acute respiratory syndrome coronavirus 2 molecular diagnostics during the COVID-19 pandemic

Lesley E. Scott, Lara D. Noble, Ashika Singh-Moodley, Trish Kahamba, Diana R. Hardie, Wolfgang Preiser, Wendy S. Stevens
African Journal of Laboratory Medicine | Vol 11, No 1 | a1429 | DOI: https://doi.org/10.4102/ajlm.v11i1.1429 | © 2022 Lesley Erica Scott, Lara Dominique Noble, Ashika Singh-Moodley, Trish Kahamba, Diana Ruth Hardie, Wolfgang Preiser, Wendy Susan Stevens | This work is licensed under CC Attribution 4.0
Submitted: 15 October 2020 | Published: 26 April 2022

About the author(s)

Lesley E. Scott, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Lara D. Noble, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Ashika Singh-Moodley, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; and, National Priority Programme, National Laboratory Services, Johannesburg, South Africa
Trish Kahamba, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa
Diana R. Hardie, Division of Medical Virology, Faculty of Health Sciences, University of Cape Town, Cape Town, South Africa
Wolfgang Preiser, Division of Medical Virology, Faculty of Medicine and Health Sciences, Stellenbosch University, Cape Town, South Africa; and, National Health Laboratory Service, Cape Town, South Africa
Wendy S. Stevens, Department of Molecular Medicine and Haematology, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; and, National Priority Programme, National Laboratory Services, Johannesburg, South Africa


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Abstract

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Keywords

SARS-CoV-2; COVID-19; molecular; RT-PCR; evaluation; assay accuracy

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