Brief Report

Characterisation of genes encoding for extended spectrum β-lactamase in Gram-negative bacteria causing healthcare-associated infections in Mwanza, Tanzania

Jenipher G. Mwakyabala, Conjester I. Mtemisika, Stacy Mshana, Adam A. Mwakyoma, Vitus Silago
African Journal of Laboratory Medicine | Vol 12, No 1 | a2107 | DOI: https://doi.org/10.4102/ajlm.v12i1.2107 | © 2023 Jenipher | This work is licensed under CC Attribution 4.0
Submitted: 30 October 2022 | Published: 12 April 2023

About the author(s)

Jenipher G. Mwakyabala, Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, United Republic of Tanzania
Conjester I. Mtemisika, Molecular Biology Laboratory, Central Pathology Laboratory, Bugando Medical Centre, Mwanza, United Republic of Tanzania
Stacy Mshana, Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, United Republic of Tanzania
Adam A. Mwakyoma, Department of Clinical Microbiology, Kilimanjaro Christian Medical Centre, Moshi, United Republic of Tanzania
Vitus Silago, Department of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza, United Republic of Tanzania

Abstract

Healthcare-associated infections (HCAIs) caused by extended spectrum β-lactamase-producing Gram-negative bacteria (ESBL-GNB) increase morbidity and mortality. This cross-sectional study characterised ESBL genes (blaCTX-MblaTEM and blaSHV) among 30 ceftriaxone-resistant GNB causing HCAIs between January 2022 and July 2022 by multiplex polymerase chain reaction assay at the zonal referral hospital in Mwanza, Tanzania. Twenty-five (83.3%) had at least one ESBL gene, of which 23/25 (92.0%) carried the blaCTX-M gene. Seventy-two percent (18/25) of the GNB-ESBL isolates carried more than one ESBL gene, of which the majority (88.8%; n = 16/25) carried the blaCTX-M and blaTEM genes. Extended spectrum β-lactamase genes, particularly blaCTX-M, are common among ceftriaxone-resistant GNB causing HCAIs.

What this study adds: This study revealed the distribution of genes (blaCTX-MblaTEM and blaSHV) coding for ESBL production among ceftriaxone resistant GNB causing HCAIs However, all ESBL producing GNB were susceptible towards ceftriaxone-sulbactam indicating that ceftriaxone-sulbactam may be empirically prescribed for treating patients with HCAIs.


Keywords

beta lactamases; Gram-negative bacteria; healthcare associated infections; multiplex PCR assay

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Crossref Citations

1. Patterns and outcomes of health-care associated infections in the medical wards at Bugando medical centre: a longitudinal cohort study
Maliha I. Kassam, Vitus Silago, Prisca Damiano, Bahati Wajanga, Jeremiah Seni, Stephen E. Mshana, Samuel Kalluvya
Antimicrobial Resistance & Infection Control  vol: 12  issue: 1  year: 2023  
doi: 10.1186/s13756-023-01345-6