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Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda

Sam L. Nsobya, Paul C. Hewett, Sam Kalibala, Barbara S. Mensch
African Journal of Laboratory Medicine | Vol 5, No 1 | a429 | DOI: https://doi.org/10.4102/ajlm.v5i1.429 | © 2016 Sam L. Nsobya, Paul C. Hewett, Sam Kalibala, Barbara S. Mensch | This work is licensed under CC Attribution 4.0
Submitted: 24 February 2016 | Published: 16 August 2016

About the author(s)

Sam L. Nsobya, Department of Pathology, School of Biomedical Science, Makerere University, Kampala, Uganda
Paul C. Hewett, Population Council, Washington, District of Columbia, United States
Sam Kalibala, Population Council, Washington, District of Columbia, United States
Barbara S. Mensch, Population Council, New York, New York, United States


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Abstract

This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS.


Keywords

HSV-2; Herpes Simplex Virus Type 2; dried blood spots; DBS; Kalon

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