Email this article 
Hide
Show all
Brief Report
Performance of Kalon herpes simplex virus 2 assay using dried blood spots among young women in Uganda
Submitted: 24 February 2016 | Published: 16 August 2016
About the author(s)
Sam L. Nsobya, Department of Pathology, School of Biomedical Science, Makerere University, Kampala, UgandaPaul C. Hewett, Population Council, Washington, District of Columbia, United States
Sam Kalibala, Population Council, Washington, District of Columbia, United States
Barbara S. Mensch, Population Council, New York, New York, United States
Abstract
This study evaluated the performance of the Kalon Biological HSV2 IgG enzyme-linked immunosorbent assay (Kalon Biological Ltd, Surrey, United Kingdom) on dried blood spots (DBS) of various dilutions compared with plasma from young women aged 18–24 years in Uganda. We estimated the sensitivity and specificity of three DBS dilutions using plasma as the reference. All three evaluated DBS dilutions yielded low sensitivities and specificities, with DBS 1:2 yielding the highest concurrence. Other herpes simplex virus type 2 assays should be examined with regard to their utility for testing DBS.
Keywords
Metrics
Total abstract views: 7098Total article views: 8054
Crossref Citations
1. Validation of Dried Blood Spot Sample Modifications to Two Commercially Available Covid-19 Igg Antibody Immunoassays
Theodore T Zava, David T Zava
Bioanalysis vol: 13 issue: 1 first page: 13 year: 2021
doi: 10.4155/bio-2020-0289
2. Twenty years of herpes simplex virus type 2 (HSV-2) research in low-income and middle-income countries: systematic evaluation of progress made in addressing WHO prioritiesfor research in HSV-2 epidemiology and diagnostics
Muna Jama, Ela Mair Owen, Belinder Nahal, Angela Obasi, Emily Clarke
BMJ Global Health vol: 9 issue: 7 first page: e012717 year: 2024
doi: 10.1136/bmjgh-2023-012717
3. Performance of ELISA and Western blot to detect antibodies against HSV-2 using dried blood spots
Santa García-Cisneros, Miguel Á. Sánchez-Alemán, Carlos J. Conde-Glez, Sergio J. Lara-Zaragoza, Antonia Herrera-Ortiz, Tanya Plett-Torres, Maria Olamendi-Portugal
Journal of Infection and Public Health vol: 12 issue: 2 first page: 224 year: 2019
doi: 10.1016/j.jiph.2018.10.007
4. Does schooling protect sexual health? The association between three measures of education and STIs among adolescents in Malawi
Barbara S. Mensch, Monica J. Grant, Erica Soler-Hampejsek, Christine A. Kelly, Satvika Chalasani, Paul C. Hewett
Population Studies vol: 74 issue: 2 first page: 241 year: 2020
doi: 10.1080/00324728.2019.1656282