Brief Report

Whole genome sequencing for drug resistance determination in Mycobacterium tuberculosis

Shaheed V. Omar, Lavania Joseph, Halima M. Said, Farzana Ismail, Nabila Ismail, Thabisile L. Gwala, Nazir A. Ismail
African Journal of Laboratory Medicine | Vol 8, No 1 | a801 | DOI: https://doi.org/10.4102/ajlm.v8i1.801 | © 2019 Shaheed V. Omar, Lavania Joseph, Halima M. Said, Farzana Ismail, Nabila Ismail, Thabisile L. Gwala, Nazir A. Ismail | This work is licensed under CC Attribution 4.0
Submitted: 07 March 2018 | Published: 21 February 2019

About the author(s)

Shaheed V. Omar, Centre for Tuberculosis, World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa
Lavania Joseph, Centre for Tuberculosis, World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa
Halima M. Said, Centre for Tuberculosis, World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa; and, Department of Medical Microbiology, University of the Free State, Bloemfontein, South Africa
Farzana Ismail, Centre for Tuberculosis, World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa; and, Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa
Nabila Ismail, Centre for Tuberculosis, World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa
Thabisile L. Gwala, Centre for Tuberculosis, World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa
Nazir A. Ismail, Centre for Tuberculosis, World Health Organization TB Supranational Reference Laboratory Network, National Institute for Communicable Diseases, National Health Laboratory Service, Johannesburg, South Africa; and, Department of Medical Microbiology, University of Pretoria, Pretoria, South Africa


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Abstract

South Africa remains challenged with a high tuberculosis burden accompanied by an increase in drug resistant cases. We assessed the use of the Illumina MiSeq, a next-generation sequencing platform for whole genome sequencing, followed by bioinformatic analysis using a commercial software package to determine resistance to selected drugs used for Mycobacterium tuberculosis treatment in our setting. Whole genome sequencing shows potential as a diagnostic platform for the detection of drug resistance in Mycobacterium tuberculosis with the provision of information for several drugs simultaneously.

Keywords

whole genome sequence; drug resistance; tuberculosis

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Crossref Citations

1. In Vitro Study of Stepwise Acquisition of rv0678 and atpE Mutations Conferring Bedaquiline Resistance
Nabila Ismail, Nazir A. Ismail, Shaheed V. Omar, Remco P. H. Peters
Antimicrobial Agents and Chemotherapy  vol: 63  issue: 8  year: 2019  
doi: 10.1128/AAC.00292-19