Brief Report

Cross-validation of a high-performance liquid chromatography nevirapine plasma assay in a resource-limited setting in Zimbabwe

Faithful Makita-Chingombe, Anthony T. Podany, Timothy Mykris, Farai Muzambi, Richard W. Browne, Andrew J. Ocque, Robin DiFrancesco, Lee C. Winchester, Courtney V. Fletcher, Tinashe Mudzviti, Charles C. Maponga, Gene D. Morse
African Journal of Laboratory Medicine | Vol 10, No 1 | a1264 | DOI: https://doi.org/10.4102/ajlm.v10i1.1264 | © 2021 Faithful Makita-Chingombe | This work is licensed under CC Attribution 4.0
Submitted: 08 May 2020 | Published: 08 July 2021

About the author(s)

Faithful Makita-Chingombe, International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe
Anthony T. Podany, Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska, United States
Timothy Mykris, Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska, United States
Farai Muzambi, International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe
Richard W. Browne, Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York, United States
Andrew J. Ocque, Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York, United States
Robin DiFrancesco, Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York, United States
Lee C. Winchester, Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska, United States
Courtney V. Fletcher, Antiviral Pharmacology Laboratory, University of Nebraska Medical Center, Omaha, Nebraska, United States
Tinashe Mudzviti, International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe
Charles C. Maponga, International Pharmacology Specialty Laboratory, School of Pharmacy, University of Zimbabwe College of Health Sciences, Harare, Zimbabwe; and, Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York, United States
Gene D. Morse, Translational Pharmacology Research Core, Center for Integrated Global Biomedical Sciences, School of Pharmacy and Pharmaceutical Sciences, University at Buffalo, Buffalo, New York, United States


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Abstract

An international HIV pharmacology specialty laboratory (PSL) was established at the University of Zimbabwe to increase bioanalytical and investigator capacities. Quantitation of plasma nevirapine in samples from the AIDS Clinical Trials Group protocol 5279 was compared between the University of Nebraska Medical Center PSL and the University of Zimbabwe PSL. Both PSLs employed internally developed methods utilising reverse-phase high-performance liquid chromatography with ultraviolet detection. Eighty-seven percent of the cross-validation results exhibited ± 20% difference.

Keywords

cross-validation; HIV; root-cause analysis; high-performance liquid chromatography

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