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Retrospective analysis of Vitek®2 performance compared to manual broth micro-dilution for colistin susceptibility testing of Acinetobacter baumanniicomplex isolates in South Africa

Vuyolwethu Fadana, Teena Thomas, Nina von Knorring
African Journal of Laboratory Medicine | Vol 11, No 1 | a1597 | DOI: https://doi.org/10.4102/ajlm.v11i1.1597 | © 2022 Vuyolwethu Fadana, Teena Thomas, Nina von Knorring | This work is licensed under CC Attribution 4.0
Submitted: 29 March 2021 | Published: 28 February 2022

About the author(s)

Vuyolwethu Fadana, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; and, Department Pathology, National Health Laboratory Services, Johannesburg, South Africa
Teena Thomas, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; and, Infectious Control Services Laboratory, National Health Laboratory Services, Johannesburg, South Africa
Nina von Knorring, School of Pathology, Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa; and, Mycobacteriology Referral Laboratory, National Health Laboratory Service, Johannesburg, South Africa


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Abstract

The manual broth micro-dilution (mBMD) is the recommended reference method for colistin minimum inhibitory concentration determination; however, it is not as readily available in South Africa as the Vitek®2. This retrospective study compared the performance of Vitek®2 against mBMD in determining the colistin minimum inhibitory concentration of 337 extensively drug-resistant Acinetobacter baumannii complex isolates. Vitek®2 yielded a categorical agreement of 89%, an essential agreement of 56%, a major error rate of 8% and a very major error rate of 55%. The Vitek®2 is not an alternative to mBMD for colistin susceptibility testing.

Keywords

Acinetobacter; colistin; broth micro-dilution; Vitek®2; antimicrobial susceptibility testing

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