Brief Report

Evaluation of Xpert Carba-R for detecting carbapenemase-producing organisms in South Africa

Sanelisiwe T. Duze, Teena Thomas, Tshegofatso Pelego, Sabelle Jallow, Olga Perovic, Adriano Duse
African Journal of Laboratory Medicine | Vol 12, No 1 | a1898 | DOI: https://doi.org/10.4102/ajlm.v12i1.1898 | © 2023 Sanelisiwe T. Duze, Teena Thomas, Tshegofatso Pelego, Sabelle Jallow, Olga Perovic, Adriano Duse | This work is licensed under CC Attribution 4.0
Submitted: 24 March 2022 | Published: 05 January 2023

About the author(s)

Sanelisiwe T. Duze, Department of Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa
Teena Thomas, Department of Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa; and, Infection Control Services Laboratory, National Health Laboratory Services, Charlotte Maxeke Academic Hospital, Johannesburg, South Africa
Tshegofatso Pelego, Infection Control Services Laboratory, National Health Laboratory Services, Charlotte Maxeke Academic Hospital, Johannesburg, South Africa
Sabelle Jallow, Department of Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa; and, Centre for Healthcare-Associated Infections, Antimicrobial Resistance and Mycoses, National Institute for Communicable Diseases, Johannesburg, South Africa
Olga Perovic, Department of Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa; and, Centre for Healthcare-Associated Infections, Antimicrobial Resistance and Mycoses, National Institute for Communicable Diseases, Johannesburg, South Africa
Adriano Duse, Department of Clinical Microbiology and Infectious Diseases, Faculty of Health Sciences, School of Pathology, University of the Witwatersrand, Johannesburg, South Africa; and, Infection Control Services Laboratory, National Health Laboratory Services, Charlotte Maxeke Academic Hospital, Johannesburg, South Africa


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Abstract

This study evaluated the performance of the Xpert Carba-R assay for detecting the five common carbapenemases in carbapenemase-producing organisms in Johannesburg, South Africa between April 2021 and September 2021. The assay demonstrated 98% sensitivity and 97% specificity. It was also able to detect all the carbapenemases in double carbapenemase producers, as well as carbapenemases in non-fermenter organisms. The Xpert Carba-R assay, therefore, allows the rapid (< 1 h) and accurate identification of the common carbapenemases in pure bacterial cultures and rectal swabs. This assay can aid in the timeous institution of appropriate treatment and infection prevention and control measures.


Keywords

Enterobacterales; carbapenemase-producing Enterobacterales; carbapenemase; Xpert Carba-R; rectal screening

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