Original Research

Sentinel laboratory compliance with best practices in Burkina Faso’s antimicrobial resistance surveillance network

Dame Yenyetou, Emmanuel Zongo, Emilie Dama, Merci Muhigwa, Issouf Sanou, Charles Sawadogo, Soumaya Ouangraoua, Ibrahim Sangare, Abdoulaye Nikiema, Anicet G. Dahourou, Abdoul S. Ouedraogo
African Journal of Laboratory Medicine | Vol 13, No 1 | a2259 | DOI: https://doi.org/10.4102/ajlm.v13i1.2259 | © 2024 Dame Yenyetou, Emmanuel Zongo, Emilie Dama, Merci Muhigwa, Issouf Sanou, Charles Sawadogo, Soumaya Ouangraoua, Ibrahim Sangare, Abdoulaye Nikiema, Anicet G. Dahourou, Abdoul S. Ouedraogo | This work is licensed under CC Attribution 4.0
Submitted: 14 July 2023 | Published: 30 January 2024

About the author(s)

Dame Yenyetou, Laboratoire National de Référence des Résistances aux Antimicrobiens, Centre Hospitalier Universitaire Souro SANOU, Bobo-Dioulasso, Burkina Faso; and Laboratoire des Pathogènes Emergents et Reémergents (LaPathER), École Doctorale Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso
Emmanuel Zongo, Laboratoire National de Référence des Résistances aux Antimicrobiens, Centre Hospitalier Universitaire Souro SANOU, Bobo-Dioulasso, Burkina Faso; and Laboratoire des Pathogènes Emergents et Reémergents (LaPathER), École Doctorale Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso; and Institut de Recherche en Sciences de la Santé, Ouagadougou, Burkina Faso
Emilie Dama, US Centers for Disease Control and Prevention, Ouagadougou, Burkina Faso
Merci Muhigwa, Laboratoire National de Référence des Résistances aux Antimicrobiens, Centre Hospitalier Universitaire Souro SANOU, Bobo-Dioulasso, Burkina Faso; and Laboratoire des Pathogènes Emergents et Reémergents (LaPathER), École Doctorale Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso
Issouf Sanou, Laboratoire des Pathogènes Emergents et Reémergents (LaPathER), École Doctorale Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso; and Service des Systèmes d’Information, et de l’Évaluation de la Qualité, Centre Muraz, Ouagadougou, Burkina Faso
Charles Sawadogo, Direction des Laboratoires de Biologie Médicale, Ministère de la Santé, Ouagadougou, Burundi
Soumaya Ouangraoua, Jhpiego Burkina Faso office, Ouagadougou, Burkina Faso
Ibrahim Sangare, Laboratoire des Pathogènes Emergents et Reémergents (LaPathER), École Doctorale Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso
Abdoulaye Nikiema, Integrated Quality Laboratory Services, Ouagadougou, Burkina Faso
Anicet G. Dahourou, US Centers for Disease Control and Prevention, Ouagadougou, Burkina Faso
Abdoul S. Ouedraogo, Laboratoire National de Référence des Résistances aux Antimicrobiens, Centre Hospitalier Universitaire Souro SANOU, Bobo-Dioulasso, Burkina Faso; and Laboratoire des Pathogènes Emergents et Reémergents (LaPathER), École Doctorale Sciences de la Santé, Université Nazi BONI, Bobo-Dioulasso, Burkina Faso

Abstract

Background: Standardising procedures is the best way to harmonise and strengthen the quality of laboratory-based antimicrobial resistance surveillance. Since 2018, Burkina Faso has developed and disseminated the national manual of procedures for performing antibiotic susceptibility tests in sentinel laboratories within its national antimicrobial resistance surveillance network.

Objective: Our study aimed to assess these sentinel laboratories’ compliance with good practices for antibiotics susceptibility tests.

Methods: Four teams evaluated the antimicrobial resistance sentinel sites laboratories throughout Burkina Faso from 19 to 28 September 2022. Eighteen out of 19 sentinel laboratories were evaluated. A four-member technical committee designed and validated the evaluation tool composed of three Microsoft Excel sheets. The evaluation emphasised quality controls for culture media, antibiotic discs and compliance with antimicrobial susceptibility testing procedures by the laboratories. Excel software was used for data recording and graphs and table design. The free R software version 4.2.0 was used for descriptive statistics. An overall score below 80% was considered noncompliance.

Results: Most (83.33%) of the sentinel laboratories conducted at least one quality control activity for culture media, and 66.67% conducted at least one quality control activity for antibiotic discs. Over three-quarters (76.47%) of the laboratories were more than 80% compliant with the modified Kirby Bauer antimicrobial susceptibility testing method.

Conclusion: The evaluation revealed the noncompliance of sentinel laboratories with the national procedure manual, particularly in the quality control component.

What this study adds: This study has provided baseline data on the sentinel laboratories’ compliance with the national antimicrobial susceptibility testing procedures manual, particularly in areas performing quality control checks or meeting quality indicators for culture media and antibiotic discs.


Keywords

culture media; antibiotic susceptibility tests; quality control; sentinel laboratories; Burkina Faso

Sustainable Development Goal

Goal 3: Good health and well-being

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