Case Study

Biosafety level-2 laboratory diagnosis of Zaire Ebola virus disease imported from Liberia to Nigeria

Olumuyiwa B. Salu, Ayorinde B. James, Bamidele O. Oke, Mercy R. Orenolu, Roosevelt A. Anyanwu, Maryam A. Abdullah, Christian Happi, Jide Idris, Ismail A. Abdus-Salam, Abdul-Salam Nasidi, Folashade T. Ogunsola, Oyewale Tomori, Sunday A. Omilabu
African Journal of Laboratory Medicine | Vol 5, No 1 | a468 | DOI: https://doi.org/10.4102/ajlm.v5i1.468 | © 2016 Olumuyiwa B. Salu, Ayorinde B. James, Bamidele O. Oke, Mercy R. Orenolu, Roosevelt A. Anyanwu, Maryam A. Abdullah, Christian Happi, Jide Idris, Ismail A. Abdus-Salam, Abdul-Salam Nasidi, Folashade T. Ogunsola, Oyewale Tomori, Sunday A. Omilabu | This work is licensed under CC Attribution 4.0
Submitted: 14 April 2016 | Published: 17 October 2016

About the author(s)

Olumuyiwa B. Salu, Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos and Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria
Ayorinde B. James, Department of Biochemistry, College of Medicine, University of Lagos, Lagos and Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria
Bamidele O. Oke, Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos, Nigeria
Mercy R. Orenolu, Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria
Roosevelt A. Anyanwu, Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria
Maryam A. Abdullah, Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria
Christian Happi, African Center of Excellence for Genomics of Infectious Diseases, Redeemers University, Ede, Osun State and Department of Biological Sciences, College of Natural Sciences, Redeemers University, Ede, Osun State, Nigeria
Jide Idris, Honourable Commissioner for Health, Lagos State Ministry of Health, Alausa, Ikeja, Lagos, Nigeria
Ismail A. Abdus-Salam, Epidemiology Unit, Directorate of Disease Control, Lagos State Ministry of Health, Alausa, Ikeja, Lagos, Nigeria
Abdul-Salam Nasidi, Nigeria Center for Disease Control, Federal Ministry of Health, Abuja, Nigeria
Folashade T. Ogunsola, Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos and Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria
Oyewale Tomori, Nigerian Academy of Science, Lagos, Nigeria
Sunday A. Omilabu, Department of Medical Microbiology and Parasitology, College of Medicine, University of Lagos, Lagos and Central Research Laboratory, College of Medicine, University of Lagos, Lagos, Nigeria


Share this article

Bookmark and Share

Abstract

Introduction: Global travel is an efficient route of transmission for highly infectious pathogens and increases the chances of such pathogens moving from high disease-endemic areas to new regions. We describe the rapid and safe identification of the first imported case of Ebola virus disease in a traveler to Lagos, Nigeria, using conventional reverse transcription polymerase chain reaction (RT-PCR) in a biosafety level (BSL)-2 facility.

Case presentation: On 20 July 2014, a traveler arrived from Liberia at Lagos International Airport and was admitted to a private hospital in Lagos, with clinical suspicion of Ebola virus disease.

Methodology and Outcome: Blood and urine specimens were collected, transported to the Virology Unit Laboratory at the College of Medicine, University of Lagos, and processed under stringent biosafety conditions for viral RNA extraction. RT-PCR was set-up to query the Ebola, Lassa and Dengue fever viruses. Amplicons for pan-filoviruses were detected as 300 bp bands on a 1.5% agarose gel image; there were no detectable bands for Lassa and Dengue viral RNA. Nucleotide BLAST and phylogenetic analysis of sequence data of the RNA-dependent RNA polymerase (L) gene confirmed the sequence to be Zaire ebolavirus (EBOV/Hsap/ NGA/2014/LIB-NIG 01072014; Genbank: KM251803.1).

Conclusion: Our BSL-2 facility in Lagos, Nigeria, was able to safely detect Ebola virus disease using molecular techniques, supporting the reliability of molecular detection of highly infectious viral pathogens under stringent safety guidelines in BSL-2 laboratories. This is a significant lesson for the many under-facilitated laboratories in resource-limited settings, as is predominantly found in sub-Saharan Africa.


Keywords

Nigerian Index Case; Ebola Virus Disease (EVD); Liberia; RT-PCR; Nigeria

Metrics

Total abstract views: 1488
Total article views: 2521

 

Crossref Citations

1. Molecular confirmation and phylogeny of Lassa fever virus in Benin Republic 2014–2016
Olumuyiwa B. Salu, Ayorinde B. James, Honoré S. Bankolé, Jijoho M. Agbla, Magloire Da Silva, Fernand Gbaguidi, Christian F. Loko, Sunday A. Omilabu
African Journal of Laboratory Medicine  vol: 8  issue: 1  year: 2019  
doi: 10.4102/ajlm.v8i1.803