Email this article 
Hide
Show all
Brief Report
Failure of BACTEC™ MGIT 960™ to detect Mycobacterium tuberculosis complex within a 42-day incubation period
Submitted: 24 July 2016 | Published: 26 April 2017
About the author(s)
Sharana Mahomed, National Health Laboratory Services, Inkosi Albert Luthuli Central Hospital, Durban, KwaZulu Natal and Department of Medical Microbiology, University of KwaZulu-Natal, Durban, South AfricaNomonde R. Dlamini-Mvelase, National Health Laboratory Services, Inkosi Albert Luthuli Central Hospital, Durban, KwaZulu Natal and Department of Medical Microbiology, University of KwaZulu-Natal, Durban, South Africa
Moses Dlamini, National Health Laboratory Services, Inkosi Albert Luthuli Central Hospital, Durban, KwaZulu Natal, South Africa
Koleka Mlisana, National Health Laboratory Services, Inkosi Albert Luthuli Central Hospital, Durban, KwaZulu Natal and Department of Medical Microbiology, University of KwaZulu-Natal, Durban, South Africa
Abstract
For the optimal recovery of Mycobacterium tuberculosis from the BACTEC™ Mycobacterium Growth Indicator Tube 960™ system, an incubation period of 42–56 days is recommended by the manufacturer. Due to logistical reasons, it is common practice to follow an incubation period of 42 days. We undertook a retrospective study to document positive Mycobacterium Growth Indicator Tube cultures beyond the 42-day incubation period. In total, 98/110 (89%) were positive for M. tuberculosis complex. This alerted us to M. tuberculosis growth detection failure at 42 days.
Keywords
Metrics
Total abstract views: 5138Total article views: 11872
Crossref Citations
1. Mycobacterium avium ssp. paratuberculosis and Crohn’s Disease—Diagnostic Microbiological Investigations Can Inform New Therapeutic Approaches
John M. Aitken, Jack E. Aitken, Gaurav Agrawal
Antibiotics vol: 13 issue: 2 first page: 158 year: 2024
doi: 10.3390/antibiotics13020158
2. ¿Puedo confiar en la negatividad de mis medios líquidos para micobacterias (BACTEC™ MGIT™)?
Ramiro López-Medrano, Isabel Burgos-Asurmendi, Octavio Rivero-Lezcano
Enfermedades Infecciosas y Microbiología Clínica vol: 44 issue: 7 first page: 503221 year: 2026
doi: 10.1016/j.eimc.2026.503221
3. Molecular detection of pre-ribosomal RNAs of Mycobacterium bovis bacille Calmette-Guérin and Mycobacterium tuberculosis to enhance pre-clinical tuberculosis drug and vaccine development
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, James G. Kublin, Sean C. Murphy
Diagnostic Microbiology and Infectious Disease vol: 108 issue: 1 first page: 116106 year: 2024
doi: 10.1016/j.diagmicrobio.2023.116106
4. Isolation of viable Mycobacterium bovis from faeces of naturally infected free-ranging rural domestic cattle (Bos taurus)
Rachiel Gumbo, Tristen Lourens, Deborah M Cooke, Tanya J Kerr, Robin M Warren, Michele A Miller, Giovanni Ghielmetti, Wynand J Goosen
Journal of Applied Microbiology vol: 136 issue: 12 year: 2025
doi: 10.1093/jambio/lxaf281
5. Evaluating the Sensitivity of Different Molecular Techniques for Detecting Mycobacterium tuberculosis Complex in Patients with Pulmonary Infection
Hassan A. Hemeg, Hamzah O. Albulushi, Hani A. Ozbak, Hamza M. Ali, Emad K. Alahmadi, Yahya A. Almutawif, Sari T. Alhuofie, Rana A. Alaeq, Areej A. Alhazmi, Mustafa A. Najim, Ahmed M. Hanafy
Polish Journal of Microbiology vol: 72 issue: 4 first page: 421 year: 2023
doi: 10.33073/pjm-2023-040
6. Diagnostic Performance of GeneXpert MTB/RIF for Pulmonary and Extra-Pulmonary Tuberculosis in Morocco.
Kholoud Maafi, Ismail Nafi, Hamza Abidar, Firdaous Laariche, Marwa Nabil, Karim Souly, Souad Aboudkhil
Natural Built Social Environment Health vol: 1 issue: 6 year: 2025
doi: 10.63095/NBSEH.25.602270