Original Research

Comparison of the COBAS/Ampliprep Taqman and Amplicor HIV-1 monitor tests in Lagos, Nigeria

Oluemi S. Amoo, Idowu A. Taiwo, Olumuyiwa O. Salu, Azuka P. Okwuraiwe, Chika K. Onwuamah, Morenike A. Awe, Osaga O. Oforomeh, Daniel I. Onwujekwe, Oliver C. Ezechi, Audu R. Ajuma
African Journal of Laboratory Medicine | Vol 2, No 1 | a68 | DOI: https://doi.org/10.4102/ajlm.v2i1.68 | © 2013 Oluemi S. Amoo, Idowu A. Taiwo, Olumuyiwa O. Salu, Azuka P. Okwuraiwe, Chika K. Onwuamah, Morenike A. Awe, Osaga O. Oforomeh, Daniel I. Onwujekwe, Oliver C. Ezechi, Audu R. Ajuma | This work is licensed under CC Attribution 4.0
Submitted: 25 May 2012 | Published: 15 May 2013

About the author(s)

Oluemi S. Amoo, Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Idowu A. Taiwo, Department of Cell Biology & Genetics, University of Lagos, Akoka, Lagos, Nigeria
Olumuyiwa O. Salu, Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Azuka P. Okwuraiwe, Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Chika K. Onwuamah, Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Morenike A. Awe, Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Osaga O. Oforomeh, Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Daniel I. Onwujekwe, Clinical Sciences Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Oliver C. Ezechi, Clinical Sciences Division, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria
Audu R. Ajuma, Human Virology Laboratory, Nigerian Institute of Medical Research, Yaba, Lagos, Nigeria

Abstract

Background: The use of real-time Polymerase chain reaction (PCR) technology options is increasing in resource-limited settings because they are faster, improve assay sensitivity,have higher throughput, larger dynamic ranges and reduced rates of contamination. In 2010, UNAIDS ranked Nigeria as the second highest population of people living with HIV and AIDS (2.98 million people) in the world.

Objective: The objective of this study was to compare the analytical performances of the Amplicor HIV-1 Monitor (version 1.5) and the COBAS Ampliprep/Taqman (version 2.0) usedin monitoring HIV disease progression in HIV-infected individuals.

Method: In a cross-sectional study, HIV-1 RNA values obtained with the Amplicor HIV-1 monitor version 1.5 were compared with those of the COBAS/Ampliprep TaqMan HIV-1version 2.0 in a routine clinical setting. Between May and November 2011, 176 plasma samples collected were analysed in parallel using both techniques. Data analysis was done using statgraphics Centurion XVI and Medcalc version 12.0.

Result: The correlation coefficient for the two assays was 0.83 and the level of agreement using a Bland–Altman plot was 94.2%.

Conclusion: These findings suggest that the results from the two methods were comparable, hence the COBAS/Ampliprep Taqman version 2.0 is recommended for high-volume laboratories.


Keywords

HIV; AIDS; HIV-1 RNA; ART; PLWHA; Viral Load

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